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Electrophoretic Study of Glutathione Reductase in Human Erythrocytes and Leucocytes

Nature volume 217, pages 256–258 (1968)Cite this article

An Erratum to this article was published on 01 January 1968

Abstract

THE question of whether the activities of NADPH—or NADH—glutathione reductase are caused by a single enzyme or by two different enzymes has never been satisfactorily resolved1–4. We have devised a new method for detecting glutathione reductase (GSSGR) activity after starch gel electrophoresis. The study of subjects with an electrophoretically fast mutant glutathione reductase has enabled us to present findings which strongly favour the suggestion that glutathione reductase activities with respect to both pyridine nucleotide coenzymes reside in the same molecule. Some observations have also been made in glutathione reductase deficient and NADH-diaphorase (NADH methaemoglobin oxido-reductase) deficient subjects.

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References

  1. Beutler, E., and Yek, M. K. Y., Blood, 21, 573 (1963).

    Article  CAS  PubMed  Google Scholar 

  2. Scott, E. M., Duncan, J. W., and Ekstrand, V., J. Biol. Chem., 238, 3928 (1963).

    CAS  PubMed  Google Scholar 

  3. Eifler, R., and Wagenknech, C., Acta Biol. Med. German., 13, 674 (1964).

    CAS  PubMed  Google Scholar 

  4. Waller, H. D., City of Hope Symp. Ser., 1, Genetically Determined Abnormalities of Red Cell Metabolism (Grime and Stratton) (in the press).

  5. Kaplan, J. C., and Beutler, E., Biochem. Biophys. Res. Commun. (in the press).

  6. Long, W. K., Science, 155, 712 (1967).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Jaffé, E. R., Amer. J. Med., 41, 786 (1966).

    Article  PubMed  Google Scholar 

  8. Carson, P. E., Brewer, G. J., and Ickes, C., J. Biol. Clin. Med., 58, 804 (1961).

    Google Scholar 

  9. Löhr, G. W., and Waller, H. D., Med. Klin., 57, 1521 (1962).

    Google Scholar 

  10. Waller, H. D., Löhr, G. W., Zysno, E., Gerok, W., Voss, D., and Strauss, G., Klin. Wochen., 43, 413 (1965).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Medicine, City of Hope Medical Center, Duarte, California

    JEAN-CLAUDE KAPLAN

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  1. JEAN-CLAUDE KAPLAN
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KAPLAN, JC. Electrophoretic Study of Glutathione Reductase in Human Erythrocytes and Leucocytes. Nature 217, 256–258 (1968). https://doi.org/10.1038/217256a0

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