THE immunoglobulins G1 represent the major component of human immunoglobulins, with a basic four chain structure consisting of two heavy and two light chains joined by disulphide bridges1. (The nomenclature used is based on that recommended by the World Health Organization (1966).) The light chains are made up of two sections—an invariable C-terminal section characteristic of each type, and a variable N-terminal section specific to each clone2–4. Peptide maps of Fd fragments5,6 suggest that a similar situation holds in heavy chains. In light chains the two sections are folded, each by a single disulphide bridge, to give two loops of almost equal size—the invariable C-terminal loop and the variable N-terminal loop7. The Fc region of human G immunoglobulins includes two intrachain disulphide bridges which impose on it a ring structure, again made up of an N-terminal and a C-terminal loop, resembling the ring structure of light chains8. In this communication, we present evidence which suggests further similarities between disulphide bridges of light and heavy chains. We also present results concerning a peptide containing both the heavy-light and heavy-heavy interchain disulphide bridges.
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Cohen, S., and Porter, R. R., Adv. Immunol., 4, 287 (1964).
Hilschmann, N., and Craig, L. C., Proc. US Nat. Acad. Sci., 53, 1403 (1965).
Titani, K., Whitley, E., Avogardo, L., and Putnam, F. W., Science, 149, 1090 (1965).
Milstein, C., Biochem. J., 101, 338 (1966).
Frangione, B., and Franklin, F. C., J. Exp. Med., 122, 1 (1965).
Frangione, B., Prelli, F., and Franklin, E. C., Immunochemistry, 4, 95 (1967).
Milstein, C., Biochem. J., 101, 352 (1966).
Frangione, B., Milstein, C., and Franklin, E. C., Biochem. J. (in the press).
Pink, J. R. L., and Milstein, C., Nature, 214, 92 (1967).
Bull. Wld. Hlth. Org., 30, 447 (1964).
Press, E. M., Biochem. J., 104, 30C (1967).
Steiner, L. A., and Porter, R. R., Biochemistry (in the press).
Singer, S. J., and Doolittle, R. F., Science, 153, 13 (1966).
Hill, R. L., Delaney, R., Fellows, R. E., and Levovitz, H. E., Proc. US Nat. Acad. Sci., 56, 1762 (1966).
Pink, J. R. L., and Milstein, C., Nature, 216, 941 (1967).
About this article
Cite this article
FRANGIONE, B., MILSTEIN, C. Disulphide Bridges of Immunoglobin G1 Heavy Chains. Nature 216, 939–941 (1967). https://doi.org/10.1038/216939b0
Frontiers in Chemistry (2020)
Frontiers in Immunology (2018)
Disulfide bond characterization of endogenous IgG3 monoclonal antibodies using LC-MS: an investigation of IgG3 disulfide-mediated isoforms
Analytical Methods (2016)
International Journal of Protein Research (2009)