Abstract
MANY methods are now available for coupling enzymes and other biologically active compounds to solid supports1–4. Most involve the preliminary preparation of a special support or of a special derivative of a general support such as cellulose; for example, the carboxymethyl or p-amino-benzyl ether derivative. We have therefore sought coupling reagents which allow a more direct approach and have developed the use of cyanuric chloride and dichloro-s-triazines. The latter can be prepared as stable compounds with solubilizing groups such as carboxy–methoxy or carboxy–methylamino, which make them very convenient reagents.
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KAY, G., CROOK, E. Coupling of Enzymes to Cellulose using Chloro-s-triazines. Nature 216, 514–515 (1967). https://doi.org/10.1038/216514a0
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DOI: https://doi.org/10.1038/216514a0
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