Polystyrene Replicas for Scanning Reflexion Electron Microscopy

Abstract

THE preparation of biological material for examination in the scanning reflexion electron microscope frequently involves coating the specimen with evaporated metal. With a small, conventional vacuum coating unit it is often difficult to achieve an adequate coating without damaging the specimen by radiation from the metal source. The technique of Lingappa and Lockwood1 which has been used in this laboratory for the observation, by light microscopy, of mycelia and stomata, has been modified and successfully applied to overcome this problem.

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  1. 1

    Lingappa, B. T., and Lockwood, J. L., Phytopathol., 53, 529 (1963).

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CHAPMAN, B. Polystyrene Replicas for Scanning Reflexion Electron Microscopy. Nature 216, 1347–1348 (1967). https://doi.org/10.1038/2161347a0

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