Size Relation between a Chromatographic Spot and its Autoradiogram


IF a chromatogram has at the origin four spots, constituting a series of concentrations of a solute (C1, C3, C5, C7) with each member of the series containing an identical amount (C*) of radioisotopic solute (radiomer), colour development after chromatography gives the expected1 near-linear relation between the area of the spot and the logarithm of its concentration, but the corresponding autoradiogram shows spots of equal size (Fig. 1). This phenomenon, which seems to oppose what might be expected intuitively2,3, has a simple explanation.

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    Aronoff, S., in Techniques in Radiobiochemistry, 73 (Iowa State Univ., Ames, 1956).

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    Ponnamperuma, C., Kirk, P., Mariner, R., and Tyson, B., Nature, 202, 393 (1964).

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    Carslaw, H. S., and Jaeger, J. C., in Conduction of Heat in Solids, second ed. (Oxford Univ. Press, London, 1959).

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    Crank, J., The Mathematics of Diffusion (Oxford Univ. Press, London, 1964).

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ARONOFF, S., SUSCHNY, O. Size Relation between a Chromatographic Spot and its Autoradiogram. Nature 216, 1214–1215 (1967).

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