Abstract
STUDIES on the African pathogenic trypanosomes have been severely hindered by the failure of the infectious forms of these organisms to grow in vitro. All previous reports of the cultivation of the African trypanosomes refer to non-infectious forms resembling those found during the multiplication of the flagellates in the insect vector (Glossina sp.). Occasional infectivity of such cultures has been reported1–3. This communication describes the occurrence of a short term multiplication which occurs when trypanosomes are separated from infected blood and maintained at 37° C in the supernatant fluid over a monolayer of L-cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Trager, W., Ann. Trop. Med. Parasit., 53, 473 (1959).
Weinman, D., Trans. Roy. Soc. Trap. Med. Hyg., 54, 166 (1960).
Amrein, Y. U., Geigy, R., and Kauffmann, M., Acta Trop., 22, 193 (1965).
Williamson, J., and Rollo, I., Nature, 170, 376 (1952).
Newton, A. A., and Wildy, P., Exp.Cell Res., 16, 624 (1959).
Balis, J., Rev. Elev. Med. Vet. Pays. Trop., 19, 351 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PAGE, R. Short Term Cultivation of Trypanosoma brucei in vitro at 37° C. Nature 216, 1141–1142 (1967). https://doi.org/10.1038/2161141a0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1038/2161141a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.