Birefringence in Unfixed Lemon Fruit Nucleoli


CHEMICAL fixation of cells and tissues for microscopical analysis carries with it the stigma of artefact production1–5. Sjöstrand and Baker6 have pointed out that the best way of checking for fixation artefacts is to employ a method of fixation or preservation of cellular components which acts on a completely different principle from chemical fixation. Cytological preparations of frozen and thawed lemon fruit tissue cultured in vitro are described here which show that the optically anisotropic nucleolar material first observed in chemically fixed tissue cultured in vitro7–11 is not an artefact of chemical fixation.

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KORDAN, H., PRESTON, R. Birefringence in Unfixed Lemon Fruit Nucleoli. Nature 216, 1105–1106 (1967).

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