Letter | Published:

Growth of the Pre-erythrocytic Tissue Stages of Plasmodium berghei in Isolated, Perfused Tree Rat Liver

Abstract

RECENT improvements in the maintenance of isolated organs in perfusion systems1–4 suggested that these techniques could be used to study certain pathogens which need a specific cellular environment and an organ of choice. The malaria parasites of man and all other mammalian plasmodia undergo a primary development cycle in the parenchyma cells of the liver of their hosts before invasion of the blood takes place. We thought therefore that, successful growth of the tissue phase of the malaria parasites (commonly known as pre-erythrocytic schizogony) in isolated, perfused liver could shed some light on the nutritional requirements of plasmodia during their initial growth in the body and be of use in studies of chemotherapy and immunity. We now report the successful growth of the pre-erythrocytic tissue stages of Plasmodium berghei in isolated, perfused tree rat liver.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Miller, L. L., Bly, C. G., Watson, M. L., and Bale, W. F., J. Exp. Med., 99, 431 (1951).

  2. 2

    Miller, L. L., and Bale, W. F., J. Exp. Med., 99, 125 (1954).

  3. 3

    Miller, L. L., Burke, W. T., and Haft, D. E., in Some Aspects of Amino Acid Supplementation (Rutgers University Press, 1956).

  4. 4

    Miller, L. L., in Recent Progress in Hormone Research, 17, 539 (1961).

  5. 5

    Yoeli, M., Most, H., and Alger, N., Science, 142, 585 (1963).

  6. 6

    Folkman, J., Cole, P., and Zimmerman, S., Ann. Surg., 164, 491 (1966).

  7. 7

    Yoeli, M., Trans. Roy. Soc. Trop. Med. and Hyg., 59, 255 (1965).

  8. 8

    Yoeli, M., and Most, H., Amer. J. Trop. Med. and Hyg., 14, 700 (1965).

  9. 9

    Yoeli, M., Upmanis, R. S., Vanderberg, J., and Most, H., Military Med., 131, 900 (1966).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.