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Growth of the Pre-erythrocytic Tissue Stages of Plasmodium berghei in Isolated, Perfused Tree Rat Liver


RECENT improvements in the maintenance of isolated organs in perfusion systems1–4 suggested that these techniques could be used to study certain pathogens which need a specific cellular environment and an organ of choice. The malaria parasites of man and all other mammalian plasmodia undergo a primary development cycle in the parenchyma cells of the liver of their hosts before invasion of the blood takes place. We thought therefore that, successful growth of the tissue phase of the malaria parasites (commonly known as pre-erythrocytic schizogony) in isolated, perfused liver could shed some light on the nutritional requirements of plasmodia during their initial growth in the body and be of use in studies of chemotherapy and immunity. We now report the successful growth of the pre-erythrocytic tissue stages of Plasmodium berghei in isolated, perfused tree rat liver.

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