Letter | Published:

Uptake of Large Molecular Weight Radioactive RNA by Protoplasts of Saccharomyces carlsbergensis

Naturevolume 214pages487488 (1967) | Download Citation

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Abstract

IN an attempt to demonstrate the presence and the biological activity of a specific (α-glucosidase-) messenger RNA in yeast RNA preparations, the conditions in which macromolecular RNA could be taken up by the intact yeast protoplast were investigated. No pinocytosis in yeast cells has yet been reported1; in the yeast cytoplasmic membrane, however, invaginations and vesicles—in other organisms these are thought to be the organelles responsible for pinocytosis—have been observed2–4. In order to study both uptake and biological activity, an RNA preparation isolated from protoplasts of Saccharomyces carlsbergensis, in which the enzyme αglucosidase had been induced5, was added to non-induced protoplasts.

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References

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    Holter, H., in Function and Structure in Micro-organisms (edit. by Pollock, M. R., and Richmond, M. H.) (Cambridge, 1965).

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  1. van't Hoff Laboratory, State University of Utrecht, The Netherlands

    • H. P. J. BLOEMERS
    •  & V. V. KONINGSBERGER

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https://doi.org/10.1038/214487a0

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