Abstract
DISC electrophoresis has been shown to be a rapid means of producing very high resolution separations of proteins1,2. Various histochemical methods, such as the periodic acid–Schiff and the Feulgen techniques, have been applied to the gel columns to identify various proteins after electrophoretic separation. Although the reproducibility of disc electrophoresis is quite good, it is difficult to correlate results of staining each of several replicate separations by different methods because one cannot be completely certain that each protein fraction migrated exactly the same distance in each replicate gel column, and various histochemical techniques may not show protein bands seen by others, thus making it difficult to determine relative positions of the bands.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ornstein, L., Ann. N.Y. Acad. Sci., 121, 321 (1964).
Davis, B. J., Ann. N.Y. Acad. Sci., 121, 404 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
STUYVESANT, V. Multiple Analyses on a Single Disc Electrophoretic Preparation. Nature 214, 405–407 (1967). https://doi.org/10.1038/214405a0
Issue Date:
DOI: https://doi.org/10.1038/214405a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.