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Dissociation and Recombination of a Polygalacturonase Complex during Ion Exchange Chromatography

Nature volume 213, pages 286–287 (1967)Cite this article

Abstract

PURIFICATION and characterization of extracellular microbial polygalacturonases depend on reliable techniques for separation. By ion exchange chromatography, six apparently distinct polygalacturonase components were separated from the culture filtrate of Fusarium oxysporum variety ‘Lycopersici’ on an anion exchanger1, and four polygalacturonase components were obtained from Coniothyrium diplodiella by similar methods on anion and cation exchangers2. We now report the existence of polygalacturonase complexes of different particle sizes and their interconversion during ion exchange chromatography.

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Author notes

  1. TERENCE R. SWINBURNE

    Present address: Plant Pathology Department, The Queen's University of Belfast, Belfast, N. Ireland

Authors and Affiliations

  1. Botany and Plant Pathology Department, Oregon State University, Corvallis, Oregon

    TERENCE R. SWINBURNE & MALCOLM E. CORDEN

Authors
  1. TERENCE R. SWINBURNE
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  2. MALCOLM E. CORDEN
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SWINBURNE, T., CORDEN, M. Dissociation and Recombination of a Polygalacturonase Complex during Ion Exchange Chromatography. Nature 213, 286–287 (1967). https://doi.org/10.1038/213286a0

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