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Separation of a Hydrolase from a Pectic Acid Transeliminase in Cell Extracts of a Bacillus

Abstract

IN the past few years it has become increasingly evident that the principal mechanism involved in bacterial breakdown of pectic substances is a transelimination reaction resulting in the formation of unsaturated uronides1–5. So far as we know, no one has isolated a true hydrolase from bacteria. The presence of a hydrolase, however, can be deduced from the results obtained by Preiss and Ashwell3. Based mainly on the products found, they concluded that the cell extracts of a pseudomonad must have contained both transeliminase and hydrolase activities. These investigators did not isolate the hydrolase.

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HASEGAWA, S., NAGEL, C. Separation of a Hydrolase from a Pectic Acid Transeliminase in Cell Extracts of a Bacillus. Nature 213, 207–208 (1967). https://doi.org/10.1038/213207a0

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