Letter | Published:

Interaction between Protein Sulphydryl Groups and Lipid Double Bonds in Biological Membranes

Nature volume 212, pages 199200 (08 October 1966) | Download Citation

Subjects

Abstract

EXPERIMENTS1,2 using brain microsomes as a model membrane system have demonstrated that membrane permeability is increased and ion discrimination decreased by two classes of compounds: (1) those that react with membrane sulphydryl groups (for example, p-chloromercuribenzoate), and (2) those that initiate peroxidation of membrane lipids (for example, iron plus ascorbic acid). These two classes of compounds are further related by the observations2 that lipid peroxidation increases the content of reactive sulphydryl groups in the membrane, whereas agents binding sulphydryl accelerate the onset of lipid peroxidation. Although this relationship between reactive sulphydryl groups and reactive double bonds may be secondary to other factors that are more directly related to increased membrane permeability, it is of interest to consider a possible structural and functional interaction between protein sulphydryl groups and lipid double bonds that could underlie these observations, and that could also be related to physiological and pharmacological membrane events.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , Arch. Biochem. Biophys., 110, 475 (1965).

  2. 2.

    , Arch. Biochem. Biophys., 112, 170 (1965).

  3. 3.

    , , and , Nature, 197, 878 (1963).

  4. 4.

    , , , and , in The Biochemical Aspects of Hormone Action, edit. by Eisenstein, A. (Little, Brown, Boston, 1964).

  5. 5.

    , , and , J. Gen. Physiol., 48, 617 (1965).

  6. 6.

    , Biochim. Biophys. Acta, 56, 42 (1962).

  7. 7.

    , , and , Amer. J. Physiol., 204, 541 (1963).

  8. 8.

    , and , J. Pharmacol. Exp. Therapeut., 130, 13 (1960).

  9. 9.

    , and , J. Bact., 84, 318 (1962).

  10. 10.

    , Arch. Biochem. Biophys., 106, 207 (1964).

  11. 11.

    , , , and , Biochim. Biophys. Acta, 43, 95 (1960).

  12. 12.

    , and , Proc. Soc. Exp. Biol. and Med., 115, 1099 (1964).

  13. 13.

    , Exp. Cell Res., 32, 118 (1963).

  14. 14.

    , and , Biochim. Biophys. Acta, 51, 442 (1961).

  15. 15.

    , Fed. Proc., 23, 630 (1964).

  16. 16.

    , , , and , Biochemistry, 1, 827 (1962).

  17. 17.

    , and , The Enzymes, 458 (Academic Press, New York, 1964).

  18. 18.

    , Science, 130, 1319 (1959).

  19. 19.

    , in The Proteins, edit. by Neurath, H., 1, 380 (Academic Press, New York, 1963).

  20. 20.

    , Biochemistry, 4, 28 (1965).

  21. 21.

    , and , Nature, 206, 1317 (1965).

  22. 22.

    , Canad. J. Biochem. Physiol., 40, 1287 (1962).

  23. 23.

    , , and , Proc. U.S. Nat. Acad. Sci., 50, 821 (1963).

  24. 24.

    , J. Amer. Oil Chemists' Soc., 40, 455 (1963).

  25. 25.

    , Structure and Function in Biological Membranes, 57, 94 (Holden-Day, San Francisco, 1965).

  26. 26.

    , and , Biochim. Biophys. Acta, 33, 319 (1959).

  27. 27.

    , Biochim. Biophys. Acta, 44, 109 (1960).

  28. 28.

    , and , J. Lipid Res., 6, 341 (1965).

  29. 29.

    , , and , J. Amer. Oil Chemists' Soc., 39, 290 (1962).

  30. 30.

    , , and , Izv. Fiz. Khim. Nauchn.-Issled. Inst. pri Irkutskum Gos. Univ., 5, 193 (1961); Chem. Abstr., 61, 1784a (1964).

  31. 31.

    , , and , J. Lipid Res., 4, 144 (1963).

  32. 32.

    , , and , Biochem. J., 93, 1c (1964).

  33. 33.

    , , , , , and , J. Chem. Soc., 2043 (1948).

Download references

Author information

Affiliations

  1. Department of Pharmacology, State University of New York, Upstate Medical Center, Syracuse, New York.

    • JOSEPH D. ROBINSON

Authors

  1. Search for JOSEPH D. ROBINSON in:

About this article

Publication history

Published

DOI

https://doi.org/10.1038/212199a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.