Abstract
IN the course of investigation of indigo biosynthesis in mutant strains of Schizophyllum commune, these strains were found to produce a number of interesting compounds which were released into the medium. Both the pigmented strains (130 and 2456) and the non-pigmented strains (699 and 2417) were grown either in flasks on a shaker or in a carboy with aeration. A minimal medium was used containing in each litre of distilled water: 20 g glucose, 1.5 g (NH4)2HPO4, 120 µg thiamine hydrochloride, 0.46 g KH2PO4, 1 g K2HPO4 and 0.5 g MgSO4.7H2O. After filtering through cheese cloth to remove the mycelium, the medium was brought to pH 4.0 with 0.1 N hydrochloric acid and extracted with an equal volume of ethyl acetate. A dark red material was obtained after drying the extract overnight over anhydrous sodium sulphate and concentrating it by distillation at 35° C under vacuum to about 3 ml.
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EPSTEIN, E. Identification of 1-(o-Carboxyphenylamino)-1-deoxyribulose and Anthranilic Acid in Cultures of the Basidiomycete Schizophyllum commune. Nature 211, 855–856 (1966). https://doi.org/10.1038/211855b0
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DOI: https://doi.org/10.1038/211855b0
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