Abstract
THE direct reaction of butterfat with 2,4-dinitrophenylhydrazine has resulted in the identification of a number of previously undescribed carbonyl compounds1,2 from dairy products. During the course of those investigations a 2,4-dinitrophenylhydrazone (hereafter abbreviated to DNPhydrazone) was observed with chromatographic properties similar to the aldehyde DNPhydrazones but differing in light absorption properties. Initial studies revealed that the unknown DNPhydrazone gave a positive response to the Liebermann-Burchard reaction. This communication presents some investigations in which the compound has been identified as the DNPhydrazone of Δ7-cholesten-3-one.
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References
Parks, O. W., Keeney, M., and Schwartz, D. P., J. Dairy Sci., 44, 1940 (1961).
Keeney, M., Katz, I., and Schwartz, D. P., Biochem. Biophys. Acta, 62, 615 (1962).
Schwartz, D. P., and Parks, O. W., Anal. Chem., 33, 1396 (1961).
Schwartz, D. P., Haller, H. S., and Keeney, M., Anal. Chem., 35, 2191 (1963).
Schwartz, D. P., Parks, O. W., and Keeney, M., Anal. Chem., 34, 669 (1962).
Parks, O. W., Keeney, M., Katz, I., and Schwartz, D. P., J. Lipid. Res., 5, 232 (1964).
Busch, W., Helv. Chem. Acta, 30, 1379 (1947).
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PARKS, O., SCHWARTZ, D., KEENEY, M. et al. Isolation of Δ7-Cholesten-3-one from Butterfat. Nature 210, 416–417 (1966). https://doi.org/10.1038/210416a0
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DOI: https://doi.org/10.1038/210416a0
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