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Preservation of Trachoma Agent in HeLa Cells

Abstract

UNLIKE most other members of the psittacosis-lympho-granuloma trachoma group, trachoma and inclusion blennorrhoea (TRIC) strains cannot usually be passed serially in cell cultures although they grow well in chick-embryo yolk-sac. A few TRIC strains have, however, been adapted to tissue culture, notably the T'ang strain, now designated TRIC/China/Peking-2/OT. Such strains may be useful when preparations free from contaminating yolk are needed. A limitation of the use of tissue culture strains is their lability on storage at low temperatures: survival of infective particles when frozen in tissue culture fluid is generally very poor.

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REED, S. Preservation of Trachoma Agent in HeLa Cells. Nature 210, 109–110 (1966). https://doi.org/10.1038/210109a0

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