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Growth of Foot-and-Mouth Disease Virus in Monolayer Cultures of Calf Thyroid Cells


THE most successful laboratory systems for the growth and assay of foot-and-mouth disease virus (FMDV) have been suckling mice1, cell cultures of calf and pig kidney2,3, and the cell line derived from baby hamster kidney (BHK 21)4. All these systems vary in their relative sensitivity for various strains of virus and none appears to effect an absolute determination of the titre of infective virus. Recently it has been found that, in the assay of unmodified cattle virus, higher titres are obtained in primary monolayer cultures of calf thyroid cells than in the systems enumerated and in some instances these cultures are 100–1,000-fold more sensitive. Calf thyroid cultures have been used previously in the isolation and study of the virus of malignant catarrhal fever5 and human thyroid cultures for the isolation of human entero-viruses and adenoviruses6,7.

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SNOWDON, W. Growth of Foot-and-Mouth Disease Virus in Monolayer Cultures of Calf Thyroid Cells. Nature 210, 1079–1080 (1966).

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