Abstract
PHOSPHATASES which exhibit a high specificity for the hydrolysis of nucleoside-5′-phosphates (5′-nucleotidases1) usually occur in association with non-specific alkaline phosphatases, for example, in blood serum. Methods which have been used for the estimation of the relative activities of the two enzymes in serum have depended on comparison of the rates of hydrolysis of two different phosphate substrates2, or on the specific inhibition of 5′-nucleotidase by nickel ions3; few attempts have been made to separate the two types of enzyme activity5. This communication describes the resolution of the 5′-nucleotidase and alkaline phosphatase activities of human blood serum by ion-exchange chromatography and gel-filtration.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Reis, J. L., Bull. Soc. Chim. Biol., Paris, 16, 385 (1934).
Dixon, T. F., and Purdom, M., J. Clin. Path., 7, 341 (1954).
Campbell, D. M., Biochem. J., 82, 34P (1962).
Kind, P. R. N., and King, E. J., J. Clin. Path., 7, 322 (1954).
Kowlessar, O. D., Pert, J. H., Haeffner, L. J., and Sleisenger, M. H., Proc. Soc. Exp. Biol. and Med., 100, 191 (1959).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MOSS, D. Separation of Serum 5′-Nucleotidase and Non-specific Alkaline Phosphatase Activities. Nature 209, 806–807 (1966). https://doi.org/10.1038/209806a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/209806a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.