Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

A Lymphocyte-stimulating Factor produced in vitro


Bain, Vas and Lowenstein1 have recently reported that when peripheral leucocytes of two individuals are cultured together, some of the cells enlarge and undergo mitosis. Bach and Hirschhorn2 have further shown that not only intact cells, but also extracts of leucocytes, disrupted by freezing and thawing, could stimulate cells to divide. Experiments reported in this communication demonstrate that cell-free media obtained from leucocytes in culture contain mitogenic factor(s), and suggest that these factors might be produced by the cells in vitro.

This is a preview of subscription content

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Bain, B., Vas, M. R., and Lowenstein, L., Blood, 23, 108 (1964).

    CAS  Article  Google Scholar 

  2. 2

    Bach, F., and Hirschhorn, K., Science, 143, 813 (1964).

    ADS  CAS  Article  Google Scholar 

Download references

Author information



Rights and permissions

Reprints and Permissions

About this article

Cite this article

GORDON, J., MACLEAN, L. A Lymphocyte-stimulating Factor produced in vitro. Nature 208, 795–796 (1965).

Download citation


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing