Effect of Ethylenediamine Tetraacetate on the Resistance of Pseudomonas aeruginosa to Antibacterial Agents

Abstract

Pseudomonas aeruginosa is a resistant pathogen which is dangerous in ophthalmology1, as a hospital cross-contaminant2, and as a plant pathogen3. We have used the spectrophotometric method of Brown and Richards4 to investigate the effect of disodium ethylenediamine tetraacetate (EDTA) on the action of several antibacterial agents against cultures of Ps. aeruginosa, NCTC 8203, in nutrient broth. It was found that the activity of polymyxin B sulphate, benzalkonium chloride and chlorhexidine diacetate against this organism was substantially increased in the presence of EDTA. Magnesium and calcium ions were found to block this potentiating action. EDTA was also found to potentiate the action of polymyxin against cells grown in undiluted serum.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Brown, M. R. W., Foster, J. H. S., Norton, D. A., and Richards, R. M. E., Pharm. J., 192, 8 (1964).

  2. 2

    Rogers, K. B., J. Appl. Bact., 23, 533 (1960).

  3. 3

    Hoff, J. C., and Drake, C. H., J. Bact., 80, 420 (1960).

  4. 4

    Brown, M. R. W., and Richards, R. M. E., J. Pharm. Pharmacol., Suppl., 16, 51T (1964).

  5. 5

    MacGregor, D. R., and Elliker, P. R., Canad. J. Microbiol., 4, 499 (1958).

  6. 6

    Repaske, R., Biochim. Biophys. Acta, 30, 225 (1958).

  7. 7

    Newton, B. A., J. Gen. Microbiol., 10, 491 (1954).

  8. 8

    Windsor, E., and Cronheim, G. E., Nature, 190, 263 (1961).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

BROWN, M., RICHARDS, R. Effect of Ethylenediamine Tetraacetate on the Resistance of Pseudomonas aeruginosa to Antibacterial Agents. Nature 207, 1391–1393 (1965). https://doi.org/10.1038/2071391a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.