Abstract
IN view of the successful removal of protein-containing material from carbohydrates1 and from nucleic acids2 by extraction with phenol, we have attempted by this means to remove the protein which remains associated with hyaluronic acid when the latter is prepared from ox synovial fluid by filtration3,4. Solutions were extracted at 0°–2° with phenol to give about equal volumes of the phenol and water-rich phases. Phenol was removed from the water-rich phase by extraction with diethyl ether or by dialysis.
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DENBOROUGH, M., OGSTON, A. Failure of Phenol to remove Residual Protein from Hyaluronic Acid. Nature 207, 1389 (1965). https://doi.org/10.1038/2071389a0
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DOI: https://doi.org/10.1038/2071389a0
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