THE existence of a gradient reflecting the ability to form flower buds, along the stems of tobacco plants (var. ‘Wisconsin 38’), has been previously reported1. Internode fragments, cultured on a simple medium devoid of growth substances, produce a callus which reflects this gradient in terms of its tendency to organize. Fragments isolated from the basal part of the stem produce only callus, those from the vegetative mid-part of the stem may produce 2 or 3 vegetative buds2, whereas those isolated from the flowering part of the stem form calluses producing 10 to 20 flowering buds3. The higher the fragments are cut, the greater the ratio of flower to vegetative buds per callus.
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AGHION-PRAT, D. Floral Meristem-organizing Gradient in Tobacco Stems. Nature 207, 1211 (1965). https://doi.org/10.1038/2071211a0
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