Visualization of the Nuclei of the Basal Melanocytes of the Black Guinea-pig and of Human Skin under the Bright Field Microscope

Abstract

THE nuclei of the melanocytes isolated from the black guinea-pig and from human skin by the method of Shukla, Karkun and Mukerji1 are always masked by the dense melanin present in the cytoplasm (Figs. 1A and 2B). A method to demelanize the cells and visualize the nuclei is reported here.

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References

  1. 1

    Shukla, R. C., Karkun, J. N., and Mukerji, B., Current Science, 22, 211 (1953).

  2. 2

    Findley, T. W., Swern, D., and Scanlan, J. T., J. Amer. Chem. Soc., 67, 412 (1945).

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    McManus, J. F. A., and Mowry, R. W., Staining Methods, 74 (P. B. Hoeber, New York, 1960).

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    Pearse, A. G. E., Histochemistry (J. and A. Churchill, Ltd., London, 1961).

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    Shukla, R. C., Karkun, J. N., and Mukerji, B., Ind. J. Med. Res., 42, 125 (1954).

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SHUKLA, R. Visualization of the Nuclei of the Basal Melanocytes of the Black Guinea-pig and of Human Skin under the Bright Field Microscope. Nature 207, 1102–1103 (1965). https://doi.org/10.1038/2071102b0

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