Abstract
THE nuclei of the melanocytes isolated from the black guinea-pig and from human skin by the method of Shukla, Karkun and Mukerji1 are always masked by the dense melanin present in the cytoplasm (Figs. 1A and 2B). A method to demelanize the cells and visualize the nuclei is reported here.
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References
Shukla, R. C., Karkun, J. N., and Mukerji, B., Current Science, 22, 211 (1953).
Findley, T. W., Swern, D., and Scanlan, J. T., J. Amer. Chem. Soc., 67, 412 (1945).
McManus, J. F. A., and Mowry, R. W., Staining Methods, 74 (P. B. Hoeber, New York, 1960).
Pearse, A. G. E., Histochemistry (J. and A. Churchill, Ltd., London, 1961).
Shukla, R. C., Karkun, J. N., and Mukerji, B., Ind. J. Med. Res., 42, 125 (1954).
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SHUKLA, R. Visualization of the Nuclei of the Basal Melanocytes of the Black Guinea-pig and of Human Skin under the Bright Field Microscope. Nature 207, 1102–1103 (1965). https://doi.org/10.1038/2071102b0
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DOI: https://doi.org/10.1038/2071102b0
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