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Chemical Induction of Heat-reversible ‘Potential Lesions’ in Vicia faba Chromosome

Abstract

WHEN main root meristems of Vicia faba were treated twice with the alkylating agent triethylenemelamine (10−5 M TEM, 5 × 10−5 M TEM, each treatment for 30 min at 24° C) the percentage of cells with induced chromatid aberrations in the first mitosis after application of the compound was found to be dependent on the sequence of the two treatments. Significantly more aberrations were induced when the first treatment was with the higher (5 × 10−5 M) and the second with the lower (10−5 M) TEM-concentration than vice versa. Preparation of the material was according to the Feulgen-squash-technique after 2-h treatment with 0.05 per cent colchicine and fixation in alcohol/acetic acid (3 : 1).

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RIEGER, R., MICHAELIS, A. Chemical Induction of Heat-reversible ‘Potential Lesions’ in Vicia faba Chromosome. Nature 206, 741–742 (1965). https://doi.org/10.1038/206741a0

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