Comparison of Techniques for the Isolation of Root-infecting Fungi


MANY techniques have been devised to study the microflora of the root-surface. Washing techniques have been devised and used by various workers1–7 for certain specific purposes. A clear demonstration of the effectiveness of the washing techniques was provided only by Harley and Waid5. In a study of the root-surface fungal population of beech, they made serial washings in sterile water and estimated the degree of completeness of the removal of fungi by the dilution plate method. They found that most of the detachable fungal units were removed in the first 10 washes. A fairly constant number of isolations were obtained thereafter by plating 0.5-cm root fragments. These isolations were thought to represent the fungal population of the root surface living in an active mycelial state.

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SINGH, K. Comparison of Techniques for the Isolation of Root-infecting Fungi. Nature 206, 1169–1170 (1965).

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