Increased Sensitivity in the Detection of Adenine and Pyridine Nucleotides by Exposure to Ultraviolet Light at Low Temperature

Abstract

IN 1957 Szent-Györgyi described a procedure for the enhancement of fluorescence of compounds spotted on paper by cooling with solid carbon dioxide or liquid nitrogen1. Gordon and South have applied this procedure to the action of aromatic acids and other aromatic ring-containing compounds2. We have found that by observing absorption, fluorescence and delayed fluorescence on exposure to long and short wave-length ultra-violet light, NAD+ and NADH can be distinguished from each other and from ADP and ATP.

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References

  1. 1

    Szent-Györgyi, A., Science, 126, 751 (1957).

  2. 2

    Gordon, M. P., and South, D., J. Chromatog., 10, 513 (1963).

  3. 3

    Randerath, K., Nature, 194, 786 (1962).

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ELLIOTT, W., KLINGMAN, J. Increased Sensitivity in the Detection of Adenine and Pyridine Nucleotides by Exposure to Ultraviolet Light at Low Temperature. Nature 206, 1044–1045 (1965). https://doi.org/10.1038/2061044b0

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