A Method for obtaining Cell Monolayers from Tissue Surfaces using Membrane Filter

Abstract

DURING an investigation into the fate of Pasteurella pseudotuberculosis injected into the peritoneal cavities of mice and guinea-pigs, difficulty was met in obtaining leucocytes from the peritoneal surfaces of the mouse. From guinea-pigs satisfactory preparations of leucocytes were obtained by pressing clean microscope slides against the visceral surface of the peritoneal wall. These slides were then fixed by heat and stained with Leishman's stain in a similar manner to blood smears. This method proved unsatisfactory with mice even if prior injection with starch was used to produce a leucocyte exudate.