Letter | Published:

Nucleoprotein Synthesis in the New-born Mouse

Nature volume 204, page 574 (07 November 1964) | Download Citation



THE proceedings of the first World Conference on Histone Biology and Chemistry were summarized in a recent article by Bonner and Ts'o1. Part of the reported discussion dealt with the probable relationship between DNA replication and histone synthesis. It was pointed out that 5-fluorodeoxyuridine blocks DNA synthesis but allows the synthesis of histone to proceed in an essentially normal manner. This evidence was cited to support the view that DNA replication and histone synthesis are not necessarily coupled although they usually accompany one another. Similar conclusions have been reached by Plaut2, who investigated the pattern of nucleoprotein synthesis in Drosophila polytene chromosomes by means of the pulse-labelling technique. He found that the labelling patterns resulting from brief exposures to tritiated precursors of DNA (H3 thymidine) and protein (H3 lysine, H3 histidine) were distinctly different. In summary, DNA labelling was often restricted to certain sites along the chromosome, while protein labelling was found to be more or less continuous. The present report, based on the results of cytophotometric investigations, furnishes further evidence in support of the view that DNA replication and histone synthesis may not be coupled.

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  1. 1.

    , and , Science, 141, 651 (1963).

  2. 2.

    , Proc. XI Intern. Cong. Genet., 1, 108 (1963).

  3. 3.

    (unpublished observations).

  4. 4.

    , Stain Technol., 20, 45 (1945).

  5. 5.

    , and , Proc. Nat. Acad. Sci. Philad., 39, 991 (1953).

  6. 6.

    , Physiol. Zool., 23, 169 (1950).

  7. 7.

    , Lab. Invest., 1, 106 (1952).

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  1. Department of Zoology and Entomology, Iowa State University, Ames.

    • JOHN H. D. BRYAN


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