Abstract
IN 1949 Koelle and Friedenwald1 described a procedure to determine the sites of cholinesterase activity in tissues. Essentially, the method depends on the hydrolysis of acetylthiocholine, or similar esters, by the tissue enzyme and the formation at or near the site of enzymatic activity of a precipitate due to the combination of copper ions and the split thiocholine. Recently this and other methods have been used to determine the electron-microscopical localization of the enzyme in mammalian muscle2–6.
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References
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MILEDI, R. Electron-microscopical Localization of Products from Histochemical Reactions used to detect Cholinesterase in Muscle. Nature 204, 293–295 (1964). https://doi.org/10.1038/204293b0
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DOI: https://doi.org/10.1038/204293b0
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