Abstract
VITAL, molecular disperse dyes are widely used in the investigation of permeability mechanisms and structural chemical changes in the cell1. The possibility of revealing such substances by means of the electron microscope was suggested by Isenberg2 and later confirmed experimentally on preparations stained after the fixation3,4. Using an electron microscope Barrnett5 revealed the azodye vitally formed in the cell as a result of a certain histochemical reaction.
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References
Nasonov, D. N., Local Reaction of Protoplasm and Spreading Excitation (Acad. Sci. U.S.S.R., Moscow, Leningrad, 1959).
Isenberg, I., Bull. Math. Biophys., 19, 279 (1957).
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Barrnett, R. J., Anat. Rec., 133, 359 (1959).
Komissarchik, Ja. Ju., and Levin, S. V., Tsitologia (U.S.S.R.), 6, 605 (1964).
Komissarchik, Ja. Ju., and Levin, S. V., Tsitologia (U.S.S.R.), 6 (in the press).
Vickerstaff, T., The Physical Chemistry of Dyeing (London, 1950).
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KOMISSARCHIK, J., LEVIN, S. Distribution of Phthalocyanine Dye (Heliogen Blue SBL) in Ultrastructures of Vital Dyed Axons of the Crab Carcinus maenas. Nature 204, 203–205 (1964). https://doi.org/10.1038/204203b0
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DOI: https://doi.org/10.1038/204203b0
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