A Micro-slide Method permitting Autoradiography of Hæmolysin-producing Cells

Abstract

THE immune response to a single injection of sheep erythrocytes has been widely used in investigations of the primary antibody response. Recently, Jerne et al.¹ have widened the potentialities of this experimental model by a method for identification and enumeration of hæmolysin-producing cells (the agar-plaque technique). Cells from a hsemolysin-producing lymphoid organ are mixed and incubated at 37° C with sheep erythrocytes in an agar medium. After addition of complement, hæmolysis occurs in zones around cells from which antibody has diffused into the medium. A similar method has been described by Ingraham². The following modification of the agar-plague technique enables tracing of radioactive isotopes to hæmolysin-producing cells by autoradiography.