Abstract
MANY spectroscopic investigations have been reported on cytochrome c in defining its role in biochemical electron transport systems1,2. These include the ultra-violet and visible absorption measurements of Keilin and Hartree3 and the optical rotatory dispersion (ORD) data of Eichorn and Cairns4. In this laboratory, we have been investigating the application of the new technique of magneto-optical rotation spectroscopy5 to the examination of different oxidation states of the cytochrome c molecule. In this type of measurement, the optical rotations induced by an external magnetic field (Faraday effect) are obtained as a function of wave-length to provide a magneto-optical rotation (MOR) spectrum. The results for cytochrome c (Figs. 1–4) are quite pronounced and show large changes with the oxidation state of the molecule.
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References
Chance, B., J. Biol. Chem., 236, 1562 (1961).
Morton, R. K., Rev. Pure and App. Chem., 8, 161 (1958).
Keilin, D., and Hartree, E. F., Proc. Roy. Soc. B, 127, 167 (1939).
Eichorn, G. L., and Cairns, J. F., Nature, 181, 994 (1958).
Shashoua, V. E., J. Amer. Chem. Soc., 82, 5505 (1960).
Shashoua, V. E., J. Amer. Chem. Soc., 86, 2109 (1964).
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SHASHOUA, V. Magneto-optical Rotation Spectra of Cytochrome c. Nature 203, 972–973 (1964). https://doi.org/10.1038/203972a0
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DOI: https://doi.org/10.1038/203972a0
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