Abstract
SEVERAL suggestions have been made for isolating from B. pertussis the cell fraction which is responsible for immunizing man against whooping cough. Most investigators1–4 have used some form of mechanical disintegration of the cells, but the possibility must also be considered, since the protective fraction is most probably located in the cell wall5, that chemical solubilization of the outer layers of the cell may be a better approach.
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VAN HEMERT, P., VAN WEZEL, A. & COHEN, H. Preparation of Soluble Pertussis Vaccine. Nature 203, 774–775 (1964). https://doi.org/10.1038/203774a0
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DOI: https://doi.org/10.1038/203774a0
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