Abstract
THE standard practice of obtaining somatic chromosomes from root-tips1 for counting is often inconvenient, particularly for species in the wild and for perennials in the field. In grafted species, for example, cultivated top-fruit clones, it is impractical to use root-tips and it may be necessary to obtain counts from meioses in pollen mother cells. Several methods are available, however, for examining chromosomes from other meristematic tissues2 such as young leaves from vegetative tips and stipules, but these do not always prove satisfactory. Burns3 has recently provided a useful method for making preparations of somatic chromosomes from corollas in Nicotiana spp. Her schedule is somewhat lengthy, and the prepared slides are not ready for examination for three days. We have been able to shorten this schedule, and have found the modifications satisfactory with the several genera tested. The abridged technique successfully used for rapidly preparing chromosomes in corolla tissue is given here.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Darlington, C. D., and LaCour, L. F., The Handling of Chromosomes (Allen and Unwin, London, 1960).
Haskell, G., and Wills, A. B., Primer of Practical Cytology (in the press).
Burns, J. A., Tobacco Sci., 8, 1 (1964).
Dyer, A. F., Stain Tech., 38, 85 (1963).
Haskell, G., and Paterson, E. B., Genetica, 33, 52 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HASKELL, G., PATERSON, E. Quick Preparation of Corolla Chromosomes from Flower Buds. Nature 203, 673–674 (1964). https://doi.org/10.1038/203673a0
Issue Date:
DOI: https://doi.org/10.1038/203673a0
This article is cited by
-
The role of the male parent in the reproduction of Rubus
Heredity (1967)
-
Chromosome Number of a Sub-Antarctic Rubus
Nature (1966)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.