Abstract
DURING the course of investigations of the degradation of the normal and unsaturated-tetragalacturonic acids by a transeliminase1,2, it was found that unsaturated tetragalacturonic acid was attacked about 1.4 times faster than the normal tetramer. Furthermore, it was observed that the unsaturated tetramer must be almost completely converted to unsaturated digalacturonic acid since there was an approximate doubling (96 per cent increase) in the optical density. All activities were determined by measuring the change in optical density at 232 mµ. Unsaturated galacturonic acid does not absorb at this wave-length, presumably because of isomerization to the keto-deoxy derivative3. The observation with unsaturated tetragalacturonic acid was confirmed with the aid of paper chromatography using the ethyl acetate–pyridine–water–acetic acid solvent1. Only a spot with an RF identical to unsaturated digalacturonic acid was detected.
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References
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Nagel, C. W., and Vaughn, R. H., Arch. Biochem. Biophys., 93, 344 (1961).
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ANDERSON, M., NAGEL, C. Effect of the Unsaturated Bond on the Degradation of the Tetragalacturonic Acids by a Transeliminase. Nature 203, 649 (1964). https://doi.org/10.1038/203649a0
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DOI: https://doi.org/10.1038/203649a0
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