Abstract
Bollum and Potter1 reported the incorporation of tritiated thymidine into DNA by enzymes present in a soluble high-speed supernatant fraction of aqueous homogenates of regenerating liver and of rat thymus. These enzyme activities were compared in nuclear and cytoplasmic aqueous extracts from Ehrlich ascites tumour cells by Smellie, Keir and Davidson2 and by Smellie and Eason3, who found them to be higher in the cytoplasmic fractions. In view of the paradox thus arising from the concept of synthesis of a nuclear component, DNA, by an enzyme apparently present in the cytoplasmic fraction, Keir, Smellie and Siebert4 re-investigated this problem with revised assay techniques. They found DNA polymerase (DNA nucleotidyltransferase) activity higher in nuclei than in cytoplasm when these were isolated in non-aqueous solvents. Behki and Schneider5 have reported similar findings in regenerating liver and Novikoff hepatoma nuclei isolated with non-aqueous solvents.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Bollum, F. J., and Potter, Van R., J. Biol. Chem., 233, 478 (1958).
Smellie, R. M. S., Keir, H. M., and Davidson, J. N., Biochim. Biophys. Acta, 35, 389 (1959).
Smellie, R. M. S., and Eason, R., Biochem. J., 80, 39P (1961).
Keir, H. M., Smellie, R. M. S., and Siebert, G., Nature, 196, 752 (1962).
Behki, R. M., and Schneider, W. C., Biochim. Biophys. Acta, 68, 34 (1963).
Lehman, I. R., Bessman, M. J., Simms, E. S., and Kornberg, A., J. Biol. Chem., 233, 162 (1958).
Walwick, E. R., and Main, R. K., Biochim. Biophys. Acta, 61, 876 (1962).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Main, R. K., and Walwick, E. R., Biochem. Biophys. Res. Commun., 4, 52 (1961).
Schneider, R. M., and Petermann, M. L., Cancer Res., 10, 751 (1950).
Hogeboom, G. H., Schneider, W. C., and Striebich, M. J., J. Biol. Chem., 196, 111 (1952).
Frick, G., Exp. Cell Res., 15, 191 (1958).
Davis, jun., W. E., and Cole, L. J., Rad. Res., 14, 104 (1961).
Billen, D., Biochim. Biophys. Acta, 68, 342 (1963).
Mazia, D., and Hinegardner, R. T., Proc. U.S. Nat. Acad. Sci., 50, 148 (1963); J. Cell. Comp. Physiol., Supp. 1, 62, 123 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MAIN, R., COLE, L. DNA Polymerase in Thymus Nuclear Fractions isolated with Ca++. Nature 203, 646–648 (1964). https://doi.org/10.1038/203646a0
Issue Date:
DOI: https://doi.org/10.1038/203646a0
This article is cited by
-
In situ detection and characterization of DNA polymerase activities in the nucleus of eukaryotic cells
Chromosoma (1977)
-
Model for DNA Polymerase
Nature (1968)
-
Altered Properties of Deoxyribonucleic Acid Nucleotidyltransferase after Infection of Mammalian Cells with Herpes Simplex Virus
Nature (1966)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.