Abstract
Bollum and Potter1 reported the incorporation of tritiated thymidine into DNA by enzymes present in a soluble high-speed supernatant fraction of aqueous homogenates of regenerating liver and of rat thymus. These enzyme activities were compared in nuclear and cytoplasmic aqueous extracts from Ehrlich ascites tumour cells by Smellie, Keir and Davidson2 and by Smellie and Eason3, who found them to be higher in the cytoplasmic fractions. In view of the paradox thus arising from the concept of synthesis of a nuclear component, DNA, by an enzyme apparently present in the cytoplasmic fraction, Keir, Smellie and Siebert4 re-investigated this problem with revised assay techniques. They found DNA polymerase (DNA nucleotidyltransferase) activity higher in nuclei than in cytoplasm when these were isolated in non-aqueous solvents. Behki and Schneider5 have reported similar findings in regenerating liver and Novikoff hepatoma nuclei isolated with non-aqueous solvents.
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References
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MAIN, R., COLE, L. DNA Polymerase in Thymus Nuclear Fractions isolated with Ca++. Nature 203, 646–648 (1964). https://doi.org/10.1038/203646a0
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DOI: https://doi.org/10.1038/203646a0
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