Abstract
MAYER et al.1 describe the preparation of complement-fixing antigen of vacuolating agent SV-40 in monkey kidney cell cultures. Strain No. 776 of SV -40 used for the work described here was obtained from Dr. H. M. Meyer. In our attempts to prepare a satisfactory complement-fixing antigen, two types of cell cultures were used: primary African green monkey kidney (AGMK) and serially propagated BS–C–l (ref. 1). These cultures were obtained from Microbiological Associates, Inc., and maintained at 34° C on Eagle's basal medium containing 2 per cent agamma ealf serum, 100 mg of streptomycin per ml., 100 units of penicillin per ml. and 2 mM of glutamine per ml.
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References
Mayer, Harry M., Hopps, Hope E., Rogers, Nancy G., Brooks, Barbara E., Bernheim, Barbara C., Jones, William P., and Douglas, Robert D., J. Immunol., 88, 796 (1962).
Sever, J. L., J. Immunol., 88, 320 (1962).
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SCHELL, K. Production of SV-40 Complement-fixing Antigen. Nature 203, 417–418 (1964). https://doi.org/10.1038/203417b0
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DOI: https://doi.org/10.1038/203417b0
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