Abstract
WATER extraction of an acetone-dried muscle powder preparation of rabbit1 is one of the standard methods of producing a solution of globular or G-actin. Such a solution possesses no birefringence of flow and has a low specific viscosity2. On making this solution 0.1 M with respect to potassium chloride, 0.001 M with respect to Mg++ and allowing to stand, a marked birefringence of flow develops and the solution has a considerably increased specific viscosity. These changes have been classically considered to be a result of the polymerization of the G-actin, as extracted, to the fibrous or F-form.
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References
Barany, M., Barany, K., and Guba, F., Nature, 179, 818 (1957).
Szent-Györgyi, A., in Chemistry of Muscular Contraction (Academic Press, 1951).
Martinosi, A., Gouvea, M. A., and Gergely, T., J. Biol. Chem., 235, 1707 (1960).
Tsuboi, K. K., and Hayashi, T., Arch. Biochem. Biophys., 100, 313 (1963).
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GRAHAM, G. Direct Extraction of Actin in the Fibrous Form from Fish Muscle. Nature 203, 405–406 (1964). https://doi.org/10.1038/203405b0
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DOI: https://doi.org/10.1038/203405b0
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