Abstract
WITH advances in the study of gastrointestinal physiology, knowledge of functions unique to the intact organ has accumulated. To facilitate investigations at a cellular level attempts have been made to obtain a pure suspension of epithelial cells for in vitro investigations. In procedures so far reported, there is contamination of the cell suspension by mesenchymal elements1–3. Additionally, abundant flora in the postnatal gut has hampered cellular maintenance and study. The following method largely overcomes these difficulties and provides a reliable cell sample for in vitro biochemical, anatomical, and immunological analysis.
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HARRER, D., STERN, B. & REILLY, R. Removal and Dissociation of Epithelial Cells from the Rodent Gastrointestinal Tract. Nature 203, 319–320 (1964). https://doi.org/10.1038/203319a0
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DOI: https://doi.org/10.1038/203319a0
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