Abstract
THE measurement of the optical thickness of biological objects, such as cells, in the living state can yield a value for the dry weight of the object1,2. Such measurements, however, are difficult to carry out with great precision. A method has previously been described3 for making very precise measurements of path difference in a reflecting interference microscope, using the principle of phase modulation. This principle can also be applied to the transmission microscope, and an experimental test of this has been made.
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References
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Davies, H. G., and Wilkins, M. H. F., Nature, 169, 541 (1952).
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DYSON, J. Precise Optical Thickness Measurement of Biological Objects. Nature 203, 1300 (1964). https://doi.org/10.1038/2031300a0
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DOI: https://doi.org/10.1038/2031300a0
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