Abstract
MANY methods have been proposed for the determination of thiol groups in proteins involving the direct attachment of a coloured moiety to the sulphydryl groups1–7. In one case at least5, such a reaction has been used to introduce a coloured label on the SH group of a protein in order to facilitate the isolation of cysteine peptides and the determination of their amino-acid sequence. In this communication we report the synthesis and potential usefulness of two new azomercurials, 4-phenylazo-2-chloromercuriphenol (I) and 4-(p-nitro-phenylazo)-2-chloromercuriphenol (II), which appear to offer certain advantages over previously reported chromophoric reagents of this type.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Bennett, H. S., and Yphantis, D. A., J. Amer. Chem. Soc., 70, 3522 (1948).
Bennett, R. J., and Seligman, A. M., Science, 116, 323 (1952).
Horowitz, M. G., and Klotz, I. M., Arch. Biochem. Biophys., 63, 77 (1956).
Akerfeldt, S., Acta Chem. Scand., 13, 627 (1959).
Witter, A., and Tuppy, H., Biochim. Biophys. Acta, 45, 429 (1960).
Clark-Walker, G. P., and Robinson, H. C., J. Chem. Soc., 2810 (1961).
Nosoh, Y., J. Biochem. (Japan), 50, 450 (1961).
Whitmore, F. C., and Middleton, E. B., J. Amer. Chem. Soc., 43, 621 (1921).
Liener, I. E., Biochim. Biophys. Acta, 53, 332 (1961).
Bernhard, S. A., and Gutfreund, H., Biochem. J., 63, 61 (1956).
King, T. P., Yphantis, D. A., and Craig, L. C., J. Amer. Chem. Soc., 82, 3350 (1960).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
CHANG, S., LIENER, I. New Chromophoric Reagents for Labelling the Thiol Groups of Proteins. Nature 203, 1065–1067 (1964). https://doi.org/10.1038/2031065a0
Issue Date:
DOI: https://doi.org/10.1038/2031065a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
