Abstract
THE technique for culturing lymphocytes from the peripheral blood1 has been used to examine chromosome damage in man following in vivo exposure to radiation2–3. The original technique specified that the cells should be cultured for about 72 h because a large number of cells in mitosis can readily be obtained at this time. Although it is known that such cells can be seen after 42–48 h in culture4 it has been thought that the great majority of cells dividing at 72 h were in their first division in culture5. Investigations concerning this point were undertaken in 1963 on cells from blood cultures of patients treated with X-rays for ankylosing spondylitis. These investigations have shown that the length of time in culture has a considerable effect on the proportion and nature of cells with chromosome structural abnormalities.
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References
Moorhead, P. S., Nowell, P. C., Mellman, W. T., Battips, D. M., and Hungerford, D. A., Exp. Cell Res., 20, 613 (1960).
Bender, M. A., and Gooch, P. C., Rad. Res., 18, 389 (1963).
Buckton, K. E., Jacobs, P. A., Court Brown, W. M., and Doll, R., Lancet, ii, 676 (1962).
Bender, M. A., and Prescott, D. M., Exp. Cell Res., 27, 221 (1962).
Bender, M. A., and Gooch, P. C., Rad. Res., 16, 14 (1962).
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BUCKTON, K., PIKE, M. Chromosome Investigations on Lymphocytes from Irradiated Patients: Effect of Time in Culture. Nature 202, 714–715 (1964). https://doi.org/10.1038/202714a0
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DOI: https://doi.org/10.1038/202714a0
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