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Effect of Buffers on the Pectolytic Activity of Culture Filtrates of Fusarium oxysporum

Abstract

PECTOLYTIC enzymes have been implicated in the disintegration of plant tissue by a number of phytopathogenic bacteria and fungi1. One procedure to assay culture filtrates for pectolytic activity requires a demonstration of a loss of viscosity for a solution of sodium polypectate or pectin in buffer, using a viscosimeter2. This communication presents results of the effect of 4 buffers at different pH values on the pectolytic activity of culture nitrates from 7 formae speciales of Fusarium oxysporum Schl. emend. Snyder and Hansen.

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References

  1. Husain, A., and Kelman, A., Plant Pathology, edit. by Horsfall, J. G., and Dimond, A. E., 2, 144 (Academic Press, 1959).

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  2. Bell, T. A., Etchells, J. H., and Jones, E. D., U.S. Dept. Agric., ARS 72–75, 1 (1955).

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MEYER, J., GARBER, E. & SHAEFFER, S. Effect of Buffers on the Pectolytic Activity of Culture Filtrates of Fusarium oxysporum. Nature 202, 414–415 (1964). https://doi.org/10.1038/202414c0

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