Abstract
THE rapid phosphorolytic cleavage of 5-bromodeoxy-uridine (BUdR) and 5-iododeoxyuridine (IUdR) in vivo1,2 greatly limits the clinical usefulness of these thymidine analogues as antineoplastic agents3, radiosensitizers4, systemic antiviral compounds5, or detectors of malignant tissue2. Investigations in man with halogen-labelled analogues have indicated that a greater amount of BUdR is probably retained by the body than is IUdR under non-loading conditions2. We have devised a rapid and convenient method which permits one to make a direct comparison of the rate and extent of degradation of thymidine and its analogues in the same subject, and to examine the effect of loading doses, depot administration6, and the influence of 5-fluorodeoxyuridine6, or agents designed to reduce nucleoside phosphorolysis7 on the degradation of the analogues.
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Prusoff, W. H., Jaffe, J. J., and Gunther, H., Biochem. Pharmacol., 3, 110 (1960).
Kriss, J. P., Maruyama, Y., Tung, L. A., Bond, S. B., and Révész, L., Cancer Res., 23, 260 (1963).
Calabresi, P., Cardoso, S. S., Finch, S. C., Kligerman, M. M., von Essen, C. F., Chu, M. Y., and Welch, A. D., Cancer Res., 21, 550 (1961).
Richardson, G. S., Hall, T. C., Green, T. H., and Ulfelder, H., Ann. N.Y. Acad. Sci., 97, 841 (1962).
Calabresi, P., McCollum, R. W., and Welch, A. D., Nature, 197, 767 (1963).
Clifton, K. H., Szybalski, W., Heidelberger, C., Gollin, F. F., Ansfield, F. J., and Vermund, H., Cancer Res., 23, 1715 (1963).
Kriss, J. P., and Bond, S. B., Biochemical Pharmacology, 13, 365 (1964).
Schulman, M. P., Metabolic Pathways, edit. by Greenberg, D. M., 2, 439 (Academic Press, New York and London, 1961).
Intern. Comm. Radiological Protection Rep. Committee II on Permissible Dose for Internal Radiation (1959) (Pergamon Press, London, 1960).
Kriss, J. P., and Révész, L., Cancer Res., 22, 254 (1962).
Djordjevic, D., and Szybalski, W., J. Exp. Med., 112, 509 (1960).
Kaufman, H. E., Proc. Soc. Exp. Biol. Med., 109, 251 (1962).
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KRISS, J., SHAW, R., LOEVINGER, R. et al. Measurement of the in vivo Degradation of Carbon-14-labelled Thymidine and Its Halogenated Analogues in Man by Continuous Monitoring of Expired Carbon-14-Carbon Dioxide. Nature 202, 1021 (1964). https://doi.org/10.1038/2021021a0
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DOI: https://doi.org/10.1038/2021021a0
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