Abstract
THE reproduction of RNA-containing bacteriophages is independent of host DNA and DNA-synthesis1–4. An understanding of the underlying processes thus should lead either to the detection of a double-stranded form of RNA, which would be an analogue to the replicative form of single-stranded DNA5, or to a novel mode of single-strand replication. To check for an RNA-intermediate of phage-RNA replication, extracts were prepared from E. coli cells infected with the RNA-containing phage ƒr (refs. 6–8), On stepwise elution9 from a methylated albumin (MAK) column10, two RNA-fractions separated which were not detected in extracts from non-infected bacteria: one fraction, eluting at 0.70 M sodium chloride, resembled the RNA extracted from purified ƒr-particles. The other fraction, eluting at 0.60 M sodium chloride, will be designated at 0.6–RNA and was prominent by its resistance towards digestion with RNase.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Doi, R. H., and Spiegelman, S., Science, 138, 1270 (1962).
Haywood, A. M., and Sinsheimer, R. L., J. Mol. Biol., 6, 247 (1963).
Cooper, S., and Zinder, N. D., Virology, 18, 405 (1962).
Hofschneider, P. H., Z. Naturforsch., 18, b, 205 (1963).
Sinsheimer, R. L., Starman, B., Nagler, C., and Guthrie, S., J. Mol. Biol., 4, 142 (1962).
Marvin, D. A., and Hoffmann-Berling, H., Nature, 197, 517 (1963).
Hoffmann-Berling, H., Marvin, D. A., and Dürwald, H., Z. Naturforsch., 18, b (in the press).
Marvin, D. A., and Hoffmann-Berling, H., Z. Naturforsch., 18, b (in the press).
Sueoka, N., and Cheng, T.-Y., J. Mol. Biol., 4, 161 (1962).
Mandell, J. D., and Hershey, A. D., Anal. Biochem., 1, 66 (1960).
Marmur, J., J. Mol. Biol., 3, 208 (1961).
Doty, P., McGill, B., and Rice, S., Proc. U.S. Nat. Acad. Sci., 44, 432 (1958).
Spiegelman, S., Hall, B. D., and Storck, R., Proc. U.S. Nat. Acad. Sci., 47, 1135 (1961).
Geiduschek, E. P., Moohr, J. W., and Weiss, S., Proc. U.S. Nat. Acad. Sci., 48, 1078 (1962).
Doty, P., Boedtker, H., Fresco, J. R., Haselkorn, R., and Litt, M., Proc. U.S. Nat. Acad. Sci., 45, 482 (1959).
Gomatos, P. J., and Tamm, I., Proc. U.S. Nat. Acad. Sci., 49, 707 (1963).
Montagnier, L., and Sanders, F. K., Nature, 199, 664 (1963).
Chamberlin, M., Baldwin, R. L., and Berg, P., J. Mol. Biol., 7, 334 (1963).
Fraenkel-Conrat, H., Biochim. Biophys. Acta, 15, 307 (1954).
Hearst, J. E., and Vinograd, J., Proc. U.S. Nat. Acad. Sci., 47, 1005 (1961).
Guthrie, S., and Sinsheimer, R. L., Biochim. Biophys. Acta, 72, 290 (1963).
Hoffmann-Berling, H., and Mazé, R., Virology, 22, 305 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KAERNER, H., HOFFMANN-BERLING, H. Synthesis of Double-stranded RNA in RNA-phage Infected E. coli Cells. Nature 202, 1012–1013 (1964). https://doi.org/10.1038/2021012a0
Issue Date:
DOI: https://doi.org/10.1038/2021012a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.