Abstract
DURING the course of investigations into the action of X-radiation on synthesis of DNA in cultured mammalian cells, we have found an effect of low specific activity radioactive thymidine that can profoundly confuse the results of such experimentation. The action of X-radiation on DNA synthesis in HeLa S3 and Chinese hamster (DFAF–33, supplied by Dr. G. Yerganian) is such that a plot of the dose-response yields a two-component curve1,2. In order to determine if the steep component was due to pool dilution, the effect of specific activity was investigated.
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PAINTER, R., RASMUSSEN, R. A Pitfall of Low Specific Activity Radioactive Thymidine. Nature 201, 409–410 (1964). https://doi.org/10.1038/201409a0
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DOI: https://doi.org/10.1038/201409a0
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