Abstract
IT was observed by Massey and Swoboda1 that on storage at 4° of Keilin–Hartree preparations isolated by centrifugation at pH 5.4 a gradual breakdown of FAD to FMN occurs. Immediately after isolation such preparations contained up to 95 per cent of the acid-extractable flavins in the form of FAD, but this quantity decreased to about 70 per cent over a period of several days. These authors concluded that in strictly fresh Keilin–Hartree preparations and in mitochondria, within experimental error, all the acid-extractable flavin may be in the form of FAD, and suggested, therefore, that FMN enzymes isolated from these sources might be artefacts.
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SALACH, J., KEARNEY, E. & SINGER, T. Further Studies on the Labile Flavin Adenine Dinucleotide of Heart Muscle Preparations. Nature 201, 1027–1028 (1964). https://doi.org/10.1038/2011027a0
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DOI: https://doi.org/10.1038/2011027a0
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