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Analysis of Tritium-labelled Human Chromosomes and Sex Chromatin

Naturevolume 199pages930932 (1963) | Download Citation



IT has been shown1,2 that differential DNA synthesis in tissue culture may be studied by the incorporation of tritiated thymidine, followed by autoradiography of the labelled chromosomes. Grain counts have been made human chromosomes labelled at the end of DNA synthesis, but the high grain density over the late-replicating X often obliterated morphology3. Asynchronous DNA synthesis has been demonstrated within human interphase nuclei4. An area of differential labelling was shown where the sex chromatin body might be expected to be present; but it was reported that the heavy concentration of grains made it difficult to determine whether an underlying body was present.

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    Gilbert, C. W., Muldal, S., Lajtha, L. G., and Rowley, J., Nature, 195, 869 (1962).

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  1. Department of Genetics, University of Sheffield, and Worksop College, Notts.

    •  & O. N. BISHOP


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